Dustmukhamedova Dinora Khamidovna Republican Specified Research-Practical Medical Center of Pediatrics 100179 Uzbekistan
Source of Support: None, Conflict of Interest: None
Aim: to study influence of phytoecdysteroids on the histological structure of the intestinal mucosa in model of chronic enteral insufficiency in the growing rats.
Materials and methods: In the experiments, there were used white pedigree less rats growing under the laboratory conditions of the National University of Uzbekistan. At rats by chronic under-eating there was induced protein-energetic insufficiency. The little rats were divided into two groups on 30 day. One subgroup received phytoecdisteroid ecdisten in dose 2,5 mg/kg for 10 days (from 30 till 40 day of life) (studied group), second subgroup received a physiological solution. Control group included healthy little rats. The rats were killed on 40 day of postnatal life. For study of histological structure of the small intestine slices of the size 0,2-0,3 cm were fixed in a solution of Karnua and 10 % solution of formalin and after the appropriate processing were filled in by paraffin. Focused slices of thickness 5-7 microns were stained by hematoxylin and eosin.
Results: The results of morphological study of histostructure of the intestinal mucosa showed that in the rats with enteral insufficiency the mucous membrane was swollen and infiltrate with single atrophic cellular elements. During treatment of enteral insufficiency with ecdisten all morphometric parameters were reliably improved (P<000,1).
Conclusions: Our data indicated that ecdisten may be used as preparation which additionally to known tonic effect has ability to stimulate protein biosynthesis in the body that results in improvement of the processes of membrane digestion and absorption in the small intestine.